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KMID : 1094720120170030538
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Biotechnology and Bioprocess Engineering
2012 Volume.17 No. 3 p.538 ~ p.546
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An application of β-glycosidase to transformation of ginsenosides for the effective production of specific ginsenosides with biological efficacy
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Her Youl
Lee Young-Chul
Oh Jin-Hwan
Choi Yoon-E
Lee Chang-Woo
Kim Jin-Suk
Kim Hwan-Mook
Yang Ji-Won
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Abstract
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Over the past several decades, the pharmacological effects of ginsenosides in Panax ginseng roots have been extensively investigated. Here, we developed a method for producing specific ginsenosides (F1 and F2) with good yields (F1:162 mg/g, F2:305 mg/g) using β-glycosidase purified from Aspergillus niger. In addition, each ginsenoside (at least 25 species) was separated and purified by high performance liquid chromatography (HPLC) using five different types of solvents and different purification steps. In addition, the Rg3:Rh2 mixture (1:1, w/w) was shown to inhibit a specific lung cancer cell line (NCI-H232) in vivo, displaying an anticancer effect at a dose lower than achieved using treatments with single Rg3 or Rh2. This finding suggests that the combination of ginsenosides for targeting anticancer is more effective than the use of a single ginsenoside from ginseng or red ginseng.
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KEYWORD
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ginsenosides, ginsenoside combination, lung cancer cell line (NCI-H23), enzymatic process, solvent system, purification
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